The main objective of the Animal House Laboratory at the Research Institute of Ophthalmology is:
- Providing professional animal support services to researchers (inside/ outside) the institute.
- The use of animals in teaching and research is an essential part of biology and medicine, and since the animals required are suitable for research and education purposes, proper care for these animals is a scientific necessity and a legal requirement.
- Achieve moral responsibility for the human care and optimal use of experimental animals.
Animal House Laboratory at the Research Institute of Ophthalmology
- It is a special nature unit, responsible for the purchase of animals, cages, feed, supplies and related materials. It is responsible for breeding, daily care, animal experimentation and general health of animals.
- The director of the unit is a veterinarian, and his main responsibility is the effective functioning of the unit and his professional qualifications and interests are in the specialty of laboratory animal medicine, which serves in all veterinary science activities and his tasks vary including administrative, clinical, educational, and research activities.
- The Unit Manager is responsible for complying with and implementing all aspects of the “Laboratory Animal Care and Application of All Guidelines” represented in the Laboratory Animal Care and Use Manual Eighth Edition.
Principles and Guidelines
Procedures followed within the unit:
Principles and Guidelines
- The Animal House Laboratory operates as a laboratory facility according to the concepts and operating procedures in the Laboratory Animals Manual and Use Publication, Eighth Edition.
- The following principles should be applied to the use and care of vertebrate animals used in testing and research as follows:
- The transport, care and use of animals should be in accordance with the publication of the Laboratory Animal Usage and Care Manual Edition VIII.
- Actions related to animals must be designed and implemented taking into account their significance to human or animal health.
- The animals selected for a procedure must be of the right choice, the right quality and the minimum number required obtaining the correct results.
- Proper use of animals, including avoiding or minimizing stress, distress and pain in the animal.
- If the procedures with animals may cause pain or distress, they should be performed with the use of an analgesic or appropriate anesthesia.
- Surgical or other painful procedures should not be performed on animals without anesthesia.
- Animals that would have suffered severe pain and the pain cannot be relieved should be euthanized at the end of the procedure or during it if possible.
- The living conditions of animals should be appropriate for their species and contribute to their health and comfort.
- Housing, feeding and care for all animals used for medical purposes must be directed by a veterinarian.
- Experimenters must be qualified and experienced to perform procedures on live animals.
Appropriate arrangements should be made for their job training, including appropriate and humane care for the use of laboratory animals
Procedures followed within the unit:
First: Acquisition of animals:
Third: Occupational Health and Safety Program – Ensure that protective clothing (such as face masks, gloves and Lab. vest) is always worn when handling animals. – Blood analysis annually. – Perform a urine analysis annually. – Immunization against tetanus.
Fourth: Selling and hosting service for experimental animals
- The number of animals and their suitability for experimentation.
- Avoid pain, stress and distress for the animals to be tested on.
- In the event of procedures that cause more than temporary, mild distress or pain to animals, sedatives, analgesics or appropriate anesthesia should be used.
- Animals are humanely euthanized after the completion of the experiment.
- In the event that animals come from outside the institute, they must be quarantined for a period (not less than 5-7 days) to ensure their health condition.
- Making a veterinary quarantine for animals coming from outside the institute for a period (not less than 5-7 days).
- Setting standards related to the space required for each animal in the event that the animals are found in very small cages or exceed their sizes in the cages that are considered suitable upon arrival; they are transferred to units of appropriate sizes.
- It is not recommended to use animal rooms for purposes other than housing animals due to the potential hazardous effects on other animals.
- The room used for experiments is available at the time specified for the start of the experiment and this room is clean and sanitized when used, as well as clean and sanitized before leaving.
- The operating room is also available at the scheduled time for the operation and is clean and sanitized when used and also before leaving.
- Smoking and eating are not allowed in any of the animal rooms.
Third: Occupational Health and Safety Program – Ensure that protective clothing (such as face masks, gloves and Lab. vest) is always worn when handling animals. – Blood analysis annually. – Perform a urine analysis annually. – Immunization against tetanus.
Fourth: Selling and hosting service for experimental animals
- For employees within the Institute:
- An application for purchasing or hosting of experimental animals is completed in the name of the researcher, his ID card number and his phone, addressing the supervisor of the Laboratory Animal House and determining the duration of the research and the purpose of use with a copy of the research protocol and the approval of the Animal Ethics Committee, then the request is submitted to the head of the institute for approval.
- Buying and selling is applied in accordance with the pricing regulations approved by the Board of Institute Directors.
- A cash supply permit is made for the Institute’s accounts with the value of the number of animals, their weight and the duration of hosting, and the money is paid by visa card.
- For sale outside the institute:
- An application for the purchase or hosting of experimental animals is completed in the name of the purchaser and his ID card number, addressing the supervisor of the Laboratory Animal House, then the application is submitted to the head of the institute for approval.
- Make an exit permit with the number of animals with a copy of the payment invoice.
- Daily cage cleaning, feeding and watering daily with routine washing and sterilization of the cage; providing clean gourmets and routine cleaning of rooms; and treating naturally occurring diseases.
- Any sick or injured animal should be humanely provided with veterinary care or culled.
- If euthanasia is done using ether, under no circumstances the carcasses should be placed in a cool room before the smell of ether in the lid disappears, and an animal should not be disposed of until it is confirmed dead.
- After the completion of the research project on animals, all animal carcasses or animal parts that are no longer needed should be placed in a plastic bag (red) and deposited in the cold room and then disposed of the animal carcasses.
- Production and supply of rats, mice and rabbits to various research bodies to contribute to the development of scientific research in accordance with international standards.
- Supply of animals to various practical faculties necessary for practical lessons for students.
- Providing a special place to conduct scientific research where the researcher buys experimental animals and then puts them in the clean room and the animal house provides the researcher with the necessary cleanliness, care and nutrition for his experimental animals for a representative payment.
- Hosting and maintenance of animals with the possibility of reviewing the scientific research protocol with the scientific team of the laboratory and considering matters such as:
- Animals suitable for experimental design.
- Animal resources and equipment.
- Post-mortem results.
- Unusual environmental requirements.
- Special diets and care.
- Pre- and post-operative care.
- Physical injuries occur from bites or scratches (rodents, rabbits). Prevention of these types of injuries by appropriate training for research staff by animal welfare staff or other qualified personnel.
- There are serious allergic risks associated with breathing or contact with allergens such as animal fur or urine. Prevention by avoiding direct exposure with the animal by always wearing protective clothing (such as face masks, gloves and a lab. vest) when handling animals.
